gelfoam sponge Search Results


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Pharmacia Upjohn LLC absorbable gelatin sponges gelfoam
Absorbable Gelatin Sponges Gelfoam, supplied by Pharmacia Upjohn LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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DuPont de Nemours gelfoam sponge
Gelfoam Sponge, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MatTek gelfoam sponge
PuMA image acquisition and automated image analysis workflow. (a) Mouse lung sections (0.5–1mm thick) containing disseminated GFP-expressing melanoma cells were placed on media-saturated <t>Gelfoam®</t> sponges cultured ex vivo for up to three weeks. Using an inverted microscope, an average of 30 non-redundant photomicrographs were recorded per experimental group every seven days. Scale bar for cell culture dish is 1 cm. Scale bar for fluorescent image is 50 μm. (b) Cross-section views of lung sections containing GFP-expressing melanoma cells within lung section atop Gelfoam® with respect to the focal plane of the image. (c) Schematic of the automated workflow used for image analysis. Output from a logistic regression model selects analyzable images for subsequent automated analysis. To standardize measurement of melanoma growth, only in-focus fluorescent cell clusters/regions of interest (ROI) were quantified. In-focus ROI were selected by applying a Gaussian gradient and selecting ROI with a clarity value above the in-focus threshold for quantification. Scale bars are 50μm.
Gelfoam Sponge, supplied by MatTek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gelfoam+sponge/gelfoam+sponge/pmc05924726-101-1-19
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Pharmacia Upjohn LLC saline-soaked gelatin sponge gelfoam
PuMA image acquisition and automated image analysis workflow. (a) Mouse lung sections (0.5–1mm thick) containing disseminated GFP-expressing melanoma cells were placed on media-saturated <t>Gelfoam®</t> sponges cultured ex vivo for up to three weeks. Using an inverted microscope, an average of 30 non-redundant photomicrographs were recorded per experimental group every seven days. Scale bar for cell culture dish is 1 cm. Scale bar for fluorescent image is 50 μm. (b) Cross-section views of lung sections containing GFP-expressing melanoma cells within lung section atop Gelfoam® with respect to the focal plane of the image. (c) Schematic of the automated workflow used for image analysis. Output from a logistic regression model selects analyzable images for subsequent automated analysis. To standardize measurement of melanoma growth, only in-focus fluorescent cell clusters/regions of interest (ROI) were quantified. In-focus ROI were selected by applying a Gaussian gradient and selecting ROI with a clarity value above the in-focus threshold for quantification. Scale bars are 50μm.
Saline Soaked Gelatin Sponge Gelfoam, supplied by Pharmacia Upjohn LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pharmacia Upjohn LLC absorbable gelatin sponges
PuMA image acquisition and automated image analysis workflow. (a) Mouse lung sections (0.5–1mm thick) containing disseminated GFP-expressing melanoma cells were placed on media-saturated <t>Gelfoam®</t> sponges cultured ex vivo for up to three weeks. Using an inverted microscope, an average of 30 non-redundant photomicrographs were recorded per experimental group every seven days. Scale bar for cell culture dish is 1 cm. Scale bar for fluorescent image is 50 μm. (b) Cross-section views of lung sections containing GFP-expressing melanoma cells within lung section atop Gelfoam® with respect to the focal plane of the image. (c) Schematic of the automated workflow used for image analysis. Output from a logistic regression model selects analyzable images for subsequent automated analysis. To standardize measurement of melanoma growth, only in-focus fluorescent cell clusters/regions of interest (ROI) were quantified. In-focus ROI were selected by applying a Gaussian gradient and selecting ROI with a clarity value above the in-focus threshold for quantification. Scale bars are 50μm.
Absorbable Gelatin Sponges, supplied by Pharmacia Upjohn LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gelfoam+sponge/absorbable+gelatin+sponge++gelfoam/10__1158_slash_1541___7786__mcr___07___2113-319-26-29
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Delasco LLC dry gelfoam collagen sponge scaffolds gelfoam
PuMA image acquisition and automated image analysis workflow. (a) Mouse lung sections (0.5–1mm thick) containing disseminated GFP-expressing melanoma cells were placed on media-saturated <t>Gelfoam®</t> sponges cultured ex vivo for up to three weeks. Using an inverted microscope, an average of 30 non-redundant photomicrographs were recorded per experimental group every seven days. Scale bar for cell culture dish is 1 cm. Scale bar for fluorescent image is 50 μm. (b) Cross-section views of lung sections containing GFP-expressing melanoma cells within lung section atop Gelfoam® with respect to the focal plane of the image. (c) Schematic of the automated workflow used for image analysis. Output from a logistic regression model selects analyzable images for subsequent automated analysis. To standardize measurement of melanoma growth, only in-focus fluorescent cell clusters/regions of interest (ROI) were quantified. In-focus ROI were selected by applying a Gaussian gradient and selecting ROI with a clarity value above the in-focus threshold for quantification. Scale bars are 50μm.
Dry Gelfoam Collagen Sponge Scaffolds Gelfoam, supplied by Delasco LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson gelfoam sponge overlayed matrigel
PuMA image acquisition and automated image analysis workflow. (a) Mouse lung sections (0.5–1mm thick) containing disseminated GFP-expressing melanoma cells were placed on media-saturated <t>Gelfoam®</t> sponges cultured ex vivo for up to three weeks. Using an inverted microscope, an average of 30 non-redundant photomicrographs were recorded per experimental group every seven days. Scale bar for cell culture dish is 1 cm. Scale bar for fluorescent image is 50 μm. (b) Cross-section views of lung sections containing GFP-expressing melanoma cells within lung section atop Gelfoam® with respect to the focal plane of the image. (c) Schematic of the automated workflow used for image analysis. Output from a logistic regression model selects analyzable images for subsequent automated analysis. To standardize measurement of melanoma growth, only in-focus fluorescent cell clusters/regions of interest (ROI) were quantified. In-focus ROI were selected by applying a Gaussian gradient and selecting ROI with a clarity value above the in-focus threshold for quantification. Scale bars are 50μm.
Gelfoam Sponge Overlayed Matrigel, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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DuPont de Nemours gelfoam sponges dupont
Photograph and line drawing of the <t>Gelfoam</t> sponge loaded in a square petri dish with a 6 by 7 grid of dots marked on surface. The stationary clamp edge is on left, and mobile plastic clip-metal bar assembly is on right.
Gelfoam Sponges Dupont, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pharmacia Upjohn LLC collagen sponge gels 1.5 cm2 gelfoam
Photograph and line drawing of the <t>Gelfoam</t> sponge loaded in a square petri dish with a 6 by 7 grid of dots marked on surface. The stationary clamp edge is on left, and mobile plastic clip-metal bar assembly is on right.
Collagen Sponge Gels 1.5 Cm2 Gelfoam, supplied by Pharmacia Upjohn LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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AmerisourceBergen Corporation gelfoam sterile compressed sponge
Photograph and line drawing of the <t>Gelfoam</t> sponge loaded in a square petri dish with a 6 by 7 grid of dots marked on surface. The stationary clamp edge is on left, and mobile plastic clip-metal bar assembly is on right.
Gelfoam Sterile Compressed Sponge, supplied by AmerisourceBergen Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TruMed LLC gelatine sponge particles gelfoam
Photograph and line drawing of the <t>Gelfoam</t> sponge loaded in a square petri dish with a 6 by 7 grid of dots marked on surface. The stationary clamp edge is on left, and mobile plastic clip-metal bar assembly is on right.
Gelatine Sponge Particles Gelfoam, supplied by TruMed LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pharmacia Upjohn LLC gelfoam thrombogenic sponge
Photograph and line drawing of the <t>Gelfoam</t> sponge loaded in a square petri dish with a 6 by 7 grid of dots marked on surface. The stationary clamp edge is on left, and mobile plastic clip-metal bar assembly is on right.
Gelfoam Thrombogenic Sponge, supplied by Pharmacia Upjohn LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


PuMA image acquisition and automated image analysis workflow. (a) Mouse lung sections (0.5–1mm thick) containing disseminated GFP-expressing melanoma cells were placed on media-saturated Gelfoam® sponges cultured ex vivo for up to three weeks. Using an inverted microscope, an average of 30 non-redundant photomicrographs were recorded per experimental group every seven days. Scale bar for cell culture dish is 1 cm. Scale bar for fluorescent image is 50 μm. (b) Cross-section views of lung sections containing GFP-expressing melanoma cells within lung section atop Gelfoam® with respect to the focal plane of the image. (c) Schematic of the automated workflow used for image analysis. Output from a logistic regression model selects analyzable images for subsequent automated analysis. To standardize measurement of melanoma growth, only in-focus fluorescent cell clusters/regions of interest (ROI) were quantified. In-focus ROI were selected by applying a Gaussian gradient and selecting ROI with a clarity value above the in-focus threshold for quantification. Scale bars are 50μm.

Journal: Clinical & experimental metastasis

Article Title: Automated Quantitative Image Analysis for ex vivo Metastasis Assays Reveals Differing Lung Composition Requirements for Metastasis Suppression by KISS1

doi: 10.1007/s10585-018-9882-1

Figure Lengend Snippet: PuMA image acquisition and automated image analysis workflow. (a) Mouse lung sections (0.5–1mm thick) containing disseminated GFP-expressing melanoma cells were placed on media-saturated Gelfoam® sponges cultured ex vivo for up to three weeks. Using an inverted microscope, an average of 30 non-redundant photomicrographs were recorded per experimental group every seven days. Scale bar for cell culture dish is 1 cm. Scale bar for fluorescent image is 50 μm. (b) Cross-section views of lung sections containing GFP-expressing melanoma cells within lung section atop Gelfoam® with respect to the focal plane of the image. (c) Schematic of the automated workflow used for image analysis. Output from a logistic regression model selects analyzable images for subsequent automated analysis. To standardize measurement of melanoma growth, only in-focus fluorescent cell clusters/regions of interest (ROI) were quantified. In-focus ROI were selected by applying a Gaussian gradient and selecting ROI with a clarity value above the in-focus threshold for quantification. Scale bars are 50μm.

Article Snippet: Each Gelfoam ® sponge containing lung sections and cancer cells were placed lung side down on a culture dish (MatTek, #P35-G-1.5-20-C) for imaging.

Techniques: Expressing, Cell Culture, Ex Vivo, Inverted Microscopy

Photograph and line drawing of the Gelfoam sponge loaded in a square petri dish with a 6 by 7 grid of dots marked on surface. The stationary clamp edge is on left, and mobile plastic clip-metal bar assembly is on right.

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Differential expression of type X collagen in a mechanically active 3-D chondrocyte culture system: a quantitative study

doi: 10.1186/1749-799X-1-15

Figure Lengend Snippet: Photograph and line drawing of the Gelfoam sponge loaded in a square petri dish with a 6 by 7 grid of dots marked on surface. The stationary clamp edge is on left, and mobile plastic clip-metal bar assembly is on right.

Article Snippet: Gelfoam sponges (Dupont, Delaware) were cut into rectangular pieces (2 cm × 2 cm), assembled in cell culture chambers, and seeded with chondrocytes as described previously [ ].

Techniques: